Enzyme-linked immunosorbent assay for rat hepatic triglyceride lipase.
A noncompetitive enzyme-linked immunosorbent assay to measure rat hepatic triglyceride lipase (H-TGL) was developed.Antibodies to rat H-TGL were Nail Lacquer purified from goat antisera by immunoadsorption on an H-TGL-Sepharose 4B column.Routinely, Immulon 2 Removawell strips were coated with the purified antibody overnight at 4 degrees C.After blo